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84 cs: Rofeocytóza
en: Ropheocytose 		4 Ondřej Zapletal, Helena Fišárková, Ondřej Zapletal, J. Kissová 10.5.2007, 15:24 29.5.2008, 14:14 29.5.2008, 14:14 Jan Blatný


Nalezené obrázky* (n=20)

AML without maturation- BM
There are numerous myeloblasts with fine nuclear chromatin, one to four nucleoli, moderately basophilic cytoplasm without granulation. No other more mature cells of granulocytic lineage are find. One cell of erythroid lineage is at 7 o'clock. / Tady jsou četné myeloblasty s jemným jaderným chromatinem, jedním až čtyřmi jadérky, středně bazofilní cytoplazmou bez granulace. Žádné další více zralé buňky granulocytární řady nejsou zjišťovány. Jedna buňka erytroidní linie je na 7. hodině. Description: There are numerous myeloblasts with fine nuclear chromatin, one to four nucleoli, moderately basophilic cytoplasm without granulation. No other more mature cells of granulocytic lineage are find. One cell of erythroid lineage is at 7 o'clock.
Type: bone marrow
Klíčová slova*: myeloblast, polychromatophilic normoblast
AML with multilineage dysplasia - BM
There are three myeloblasts in the picture, one of them underneath the centre with cleft nucleus. Three immature cells of erythroid lineage (proerythroblast and two more or less mature early normoblasts) belong to cells localized around the picture's centre. At 5 o'clock lays one dysplastic cell of perhaps granulocytic or may be monocytic lineage; it has band-like but really immature nucleus with fine chromatin and clear nucleoli and immature cytoplasm without granulation. Another almost bizarre cell is in the centre, it the most probably belongs in granulocytic series, it has moderately mature nucleus, basophilic cytoplasm almost without granulation but with one digested late normoblast. / Na obrázku jsou tři myeloblasty, jeden z nich pod centrem se štěpeným jádrem. Tři nezralé buňky erytroidní linie (proerytroblast a dva méně či více zralé časné normoblasty patří k buňkám umístěným okolo středu obrázku. Na 5. hodině leží dysplastická buňka granulocytární nebo možná monocytární řady; má tyčkovité ale opravdu nezralé jádro s jemným chromatinem a jasnými jadérky a nezralou cytoplazmu bez granulace. Jiná téměř bizarní buňka je ve středu, nejpravděpodobněji patří ke granulocytární řadě, má středně zralé jádro, basofilní cytoplazmu téměř bez granulace ale s jedním pohlceným pozdním normoblastem. Description: There are three myeloblasts in the picture, one of them underneath the centre with cleft nucleus. Three immature cells of erythroid lineage (proerythroblast and two more or less mature early normoblasts) belong to cells localized around the picture's centre. At 5 o'clock lays one dysplastic cell of perhaps granulocytic or may be monocytic lineage; it has band-like but really immature nucleus with fine chromatin and clear nucleoli and immature cytoplasm without granulation. Another almost bizarre cell is in the centre, it the most probably belongs in granulocytic series, it has moderately mature nucleus, basophilic cytoplasm almost without granulation but with one digested late normoblast.
Type: bone marrow
Klíčová slova*: myeloblast, proerythroblast, basophilic normoblast, haemophagocytosis
AML with multilineage dysplasia - BM
There are seven myeloblasts underneath the centre of the picture and three proerythroblasts at the upper left. Between these two groups of the cells lies one agranular band and one red mitosis. Two early polychromatophil red cells precursors (upper left) have megaloblastoid nuclear pattern and they are bilobated. Three late polychromatophil normoblasts (to the left and down from the centre) have pycnotic nuclei. / Pod centrem obrázku je sedm myeloblastů a tři proerytroblasty jsou vlevo nahoře. Mezi těmito dvěma skupinami buněk leží jedna agranulární tyčka a jedna červená mitóza. Dva časné polychromatofilní prekurzory erytrocytů mají megaloblastoidní vzhled jádra a jsou bilobární. Tři pozdní polychromatofilní normoblasty (doleva a dolu z centra) mají pyknotická jádra. Description: There are seven myeloblasts underneath the centre of the picture and three proerythroblasts at the upper left. Between these two groups of the cells lies one agranular band and one red mitosis. Two early polychromatophil red cells precursors (upper left) have megaloblastoid nuclear pattern and they are bilobated. Three late polychromatophil normoblasts (to the left and down from the centre) have pycnotic nuclei.
Type: bone marrow
Klíčová slova*: myeloblast, proerythroblast, polychromatophilic normoblast, megaloblastic haematopoiesis
Anaplastic large cell lymphoma - ALK negative
Another example of anaplastic large lymphoma cell. Here is ex-centric, horse-shoe-shaped nucleus. Eosinophilic area of cytoplasm is at 3 o'clock. Nuclear chromatin is fine, nucleus contains several prominent nucleoli. There is bi-lobar late normoblast with internuclear bride at 3 o'clock. / Jiný příklad buňky anaplastického velkobuněčného lymfomu. Zde je jádro excentrické, podkovovitého tvaru. Eozinofilní oblast cytoplazmy je na 3 hodině. Jaderný chromatin je jemný, jádro obsahuje několik prominujících jadérek. Na 3. hodině je pozdní normoblast, bilobární s jaderným můstkem. Description: Another example of anaplastic large lymphoma cell. Here is ex-centric, horse-shoe-shaped nucleus. Eosinophilic area of cytoplasm is at 3 o'clock. Nuclear chromatin is fine, nucleus contains several prominent nucleoli. There is bi-lobar late normoblast with internuclear bride at 3 o'clock.
Type: bone marrow
Klíčová slova*: lymphocyte atypical
Chronic myelogenous leukaemia - chronic phase
Various stages of maturation in neutrophil lineage (one myeloblast in the centre, two segments and metamyelocyte). Only one early normoblast is under the myeloblast; beside it to the left is basophil. / Různá stádia vyzrávání v neutrofilní řadě (jeden myeloblast v centru, dva segmenty a metamyelocyt). Jen jeden časný normoblast je pod myeloblastem, vedle něj doleva je basofil. Description: Various stages of maturation in neutrophil lineage (one myeloblast in the centre, two segments and metamyelocyte). Only one early normoblast is under the myeloblast; beside it to the left is basophil.
Type: bone marrow
Klíčová slova*: myeloblast, metamyelocyte, basophil, segmented granulocyte, normoblast
Chronic myelogenous leukaemia - chronic phase
Excess of granulocytic cells - the most of them are neutrophils in different stage of maturation including segmented ones, only two are eosinophils (one to the middle left and one to the middle right). Only one late erythroblast with pycnotic nucleus is underneath the centre of the picture. / Převaha buněk granulocytární řady - většina z nich jsou neutrofily v různém stadiu vývoje včetně segmentů, jen dva jsou eozinofily (jeden ze středu doleva a jeden ze středu doprava). Jen jeden pozdní erytroblast s pyknotickým jádrem je pod centrem obrázku. Description: Excess of granulocytic cells - the most of them are neutrophils in different stage of maturation including segmented ones, only two are eosinophils (one to the middle left and one to the middle right). Only one late erythroblast with pycnotic nucleus is underneath the centre of the picture.
Type: bone marrow
Klíčová slova*: eosinophil, normoblast
Chronic myelogenous leukaemia - chronic phase
Granulocytic lineage is increased and erythroid lineage is decreased in CML. There are only two early normoblasts here in the picture (at 8 and 9 o'clock). Myeloid:erhythroid ratio is 15:1 in this patient, but it can reach even value of 25:1. / Granulocytární řada je zvýšena a erytroidní řada snížena u CML. Zde jsou na obrázku jen dva časné normoblasty (na 8. a 9. hodině). U této pacientky je myeloidní:erytroidní poměr 15:1, ale může dosáhnout dokonce hodnoty 25:1. Description: Granulocytic lineage is increased and erythroid lineage is decreased in CML. There are only two early normoblasts here in the picture (at 8 and 9 o'clock). Myeloid:erhythroid ratio is 15:1 in this patient, but it can reach even value of 25:1.
Type: bone marrow
Klíčová slova*: normoblast
Chronic myeloid leukaemia, blast phase- BM
Infiltration of lymphoblasts, some of them with nuclear indentation, normoblast on the left side in the centre. / Infiltrace lymfoblasty, některé z nich se zářezem v jádře, normoblast ve středu vlevo. Description: Infiltration of lymphoblasts, some of them with nuclear indentation, normoblast on the left side in the centre.
Type: bone marrow
Klíčová slova*: lymphoblast, normoblast
CML accelerated phase - PB
Leucocytosis, left shift. Two blasts at the right side (at 3. o´clock). basophil on the left. Normoblast in the centre, lymphocyte at the right side up. / Leukocytóza, posun doleva. Dva blasty vpravo ( na 3. hodině), bazofil vlevo. Normoblast uprostřed, lymfocyt vpravo nahoře. Description: Leucocytosis, left shift. Two blasts at the right side (at 3. o´clock). basophil on the left. Normoblast in the centre, lymphocyte at the right side up.
Type: peripheral blood
Klíčová slova*: myeloblast, myelocyte, metamyelocyte, basophil, lymphocyte, normoblast
Small- cell bronchial carcinoma - BM
The last example bone marrow tumour cells' infiltration in this case - the cluster of various size tumour cells with immature appearance, narrow rim of basophilic cytoplasm and with fine chromatin and a few nucleoli in nucleus. There are two late erythroblasts at the left side of the picture. / Poslední příklad infiltrace nádorovými buňkami u tohoto případu - trs různě velkých nádorových buněk nezralého vzhledu s úzkým lemem bazofilní cytoplazmy, v jádře jemný chromatin a jadérka. Vlevo jsou dva pozdní erytroblasty. Description: The last example bone marrow tumour cells' infiltration in this case - the cluster of various size tumour cells with immature appearance, narrow rim of basophilic cytoplasm and with fine chromatin and a few nucleoli in nucleus. There are two late erythroblasts at the left side of the picture.
Type: bone marrow
Klíčová slova*: polychromatophilic normoblast, orthochromatophilic normoblast, tumour cell
Small - cell bronchial carcinoma - BM
Tumour cells' infiltration - cluster of various size immature cells with narrow cytoplasm's rim, fine chromatin and nucleoli; there is late erythroblast at the upper right and there are some naked nucleoli at the smear. / Infiltrace nádorovými buňkami - trs různě velkých buněk s úzkým lemem cytoplazmy, v jádře jemný chromatin a jadérka, v nátěru je vpravo nahoře pozdní erytroblast a několik holých jáder. Description: Tumour cells' infiltration - cluster of various size immature cells with narrow cytoplasm's rim, fine chromatin and nucleoli; there is late erythroblast at the upper right and there are some naked nucleoli at the smear.
Type: bone marrow
Klíčová slova*: orthochromatophilic normoblast, naked nucleus, tumour cell
Small- cell bronchial carcinoma - BM
Tumour cells' infiltration of the bone marrow - cluster of smaller to middle size cells with high nucleus - cytoplasm ratio, there is fine chromatin in the nucleus with one to more nucleoli. There is one hypergranular neutrophile myelocyte at left side, segmented neuthrophil with pseudo-pelgeroid appearance at the top and late erythroblast at the upper left, there are same naked nucleoli at the smear. / Infiltrace nádorovými buňkami - trs menších a středně velkých buněk s vysokým poměrem jádra a cytoplazmy, v jádře jemný chromatin s jedním až několika jadérky. Vlevo je jeden hypergranulovaný neutrofilní myelocyt, nahoře neutrofilní segment pseudo-pelgeroidního vzhledu a vpravo nahoře pozdní erytroblast, v nátěru je také několik holých jader. Description: Tumour cells' infiltration of the bone marrow - cluster of smaller to middle size cells with high nucleus - cytoplasm ratio, there is fine chromatin in the nucleus with one to more nucleoli. There is one hypergranular neutrophile myelocyte at left side, segmented neuthrophil with pseudo-pelgeroid appearance at the top and late erythroblast at the upper left, there are same naked nucleoli at the smear.
Type: bone marrow
Klíčová slova*: myelocyte, orthochromatophilic normoblast, hypergranulation, naked nucleus, tumour cell
Small- cell bronchial carcinoma - BM
The first example of the bone marrow infiltration in this case - the cluster of the middle size cells with narrow rim of basophilic cytoplasm; there is fine chromatin and some nucleoli in the nucleus. There is eosinophil metamyelocyte at the upper left, neutrophil metamyelocyte at the upper right and there are two late erythroblasts at the upper part of the picture. Naked nucleoli are the typical content of the smear. / První příklad infiltrace nádorovými buňkami u tohoto případu - trs středně velkých buněk s úzkým lemem bazofilní cytoplamy; v jádře je jemný chromatin a jadérka. Vlevo nahoře eosinofilní metamyelocyt, vpravo nahoře neutrofilní metamyelocyt, v horní části obrázku dva pozdní erytroblasty. Holá jádra jsou typickou součástí nátěru. Description: The first example of the bone marrow infiltration in this case - the cluster of the middle size cells with narrow rim of basophilic cytoplasm; there is fine chromatin and some nucleoli in the nucleus. There is eosinophil metamyelocyte at the upper left, neutrophil metamyelocyte at the upper right and there are two late erythroblasts at the upper part of the picture. Naked nucleoli are the typical content of the smear.
Type: bone marrow
Klíčová slova*: metamyelocyte, orthochromatophilic normoblast, naked nucleus, tumour cell
APL- bone marrow 4
Smear show typical pathological cells of acute promyelocytic leukamia - hypergranulated abnormal promyelocyte and "faggot cells". Rest of cells are two normoblasts and damaged cells. / Nález ukazuje typické patologické elementy akutní promyelocytární leukémie- hypergranulované abnormální promyelocyty a tzv."faggot cells". Zbytek buněk tvoří dva normoblasty a rozbité buňky. Description: Smear show typical pathological cells of acute promyelocytic leukamia - hypergranulated abnormal promyelocyte and "faggot cells". Rest of cells are two normoblasts and damaged cells.
Type: bone marrow
Klíčová slova*: faggot cell
MDS RAEB I- bone marrow
In the middle of the picture is dysplastic erythroblast with mature abundant ortochomatophilic cytoplasm and irregular shape of the nucleus. Two blast, that at 11 o'clock is myeloblast with numerous azurophilic granules. / Uprostřed obrázku je dysplastický erytroblast se zralou bohatou ortochromatofilní cytoplazmou a nepravidelným tvarem jádra. Dva blasty, ten na 11. hodině je myeloblast s početnými azurofilními granuly. Description: In the middle of the picture is dysplastic erythroblast with mature abundant ortochomatophilic cytoplasm and irregular shape of the nucleus. Two blast, that at 11 o'clock is myeloblast with numerous azurophilic granules.
Type: bone marrow
Klíčová slova*: myeloblast, erythroblast, orthochromatophilic normoblast
Erythroblastic island - Perls´ stain
see previous / viz předchozí Description: see previous
Type: bone marrow
Klíčová slova*: macrophage, normoblast
Erythroblastic island - Perls´ stain
Cumulation of iron (hemosiderin) in macrophage. / Při barvení na nehemové železo je patrná jeho kumulace v makrofágu. Description: Cumulation of iron (hemosiderin) in macrophage.
Type: bone marrow
Klíčová slova*: macrophage, normoblast
Erythroblastic island
Concentric circles of erythroblasts closely surround a macrophage, those closer to the macrophage are less mature. Macrophage sends slender processes which envelop each erythroblast. / Koncentrické kruhy erytroblastů kolem makrofágu, vnější jsou zralejší. Štíhlé výběžky cytoplasmy makrofágu obkloupují erytroblasty. Description: Concentric circles of erythroblasts closely surround a macrophage, those closer to the macrophage are less mature. Macrophage sends slender processes which envelop each erythroblast.
Type: bone marrow
Klíčová slova*: macrophage, normoblast, erythroblast
Erythroblastic island
Macrophage (in center) surrounded by cluster of mature erythroblasts. The macrophage phagocytoses defective erythroblasts and extruded erythroblast nuclei. Macrophage also serves as a "iron store" for erythroblasts. / Makrofág (siderofág) uprostřed slouží jako zásobárna Fe. Je obklopený koncentrickým prstencem vyzrávajících polychromatofilních normoblastů. Makrofág fagocytuje defektní erytroblasty a jádra, která jsou v průběhu zrání z erytroblastů vyloučena. Description: Macrophage (in center) surrounded by cluster of mature erythroblasts. The macrophage phagocytoses defective erythroblasts and extruded erythroblast nuclei. Macrophage also serves as a "iron store" for erythroblasts.
Type: bone marrow
Klíčová slova*: macrophage, normoblast, erythroblast, orthochromatophilic normoblast
MDS RAEB II
In the upper part of the picture note a myeloblast wiht Auer rods, and nucleoli. Below this cell there is a plasmatic cell and two promyelocytes. On the right there is a group of two megaloblasts and two normoblast. / V horní části obrázku leží myeloblast s několika Auerovými tyčemi, v jádře zřetelná jadérka, pod ním plasmatická buňka a dva promyelocyty. Vpravo skupina erytroblastů se 2 normoblasty a 2 megaloblasty. Description: In the upper part of the picture note a myeloblast wiht Auer rods, and nucleoli. Below this cell there is a plasmatic cell and two promyelocytes. On the right there is a group of two megaloblasts and two normoblast.
Type: bone marrow
Klíčová slova*: myeloblast, normoblast, plasma cell, megaloblast, Auer rod