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Anaplastic large cell lymphoma - ALK negative
Two closely pressed malignant lymphoma cells with ex-centric nucleus, reticular chromatin pattern, few nucleoli and abundant pale basophilic finely vacuolized cytoplasm. / Dvě těsně přitištěné maligní lymfomové buňky s excentrickým jádrem, síťovitým vzorkem jaderného chromatinu, několika jadérky a bohatou světle bazofilní jemně vakuolizovanou cytoplazmou. Description: Two closely pressed malignant lymphoma cells with ex-centric nucleus, reticular chromatin pattern, few nucleoli and abundant pale basophilic finely vacuolized cytoplasm.
Type: bone marrow
Klíčová slova*: lymphocyte atypical
Anaplastic large cell lymphoma - ALK negative
Large cell with kidney-shaped nucleus with eosinophilic region in cytoplasm at lower right quadrant of the cell, while another parts of cytoplasm is basophilic. The cytoplasm is abundant. There are some blue nucleoli in the nucleus. The cell size one can estimate to 40 micrometers and more. / Velká buňka s ledvinovitým tvarem jádra s eozinofilní oblastí v cytoplazmě v dolním pravém kvadrantu buňky. Cytoplazma je bohatá. Několik modrých jadérek je v jádře. Velikost buňky může být odhadována na 40 mikrometrů a více. Description: Large cell with kidney-shaped nucleus with eosinophilic region in cytoplasm at lower right quadrant of the cell, while another parts of cytoplasm is basophilic. The cytoplasm is abundant. There are some blue nucleoli in the nucleus. The cell size one can estimate to 40 micrometers and more.
Type: bone marrow
Klíčová slova*: lymphocyte atypical
Anaplastic large cell lymphoma - ALK negative
The large cell or one can say almost giant one tumour cell in blood marrow in ALCL and ALK negative T- cell lymphoma. Kidney shape of nucleus is rather typical as also they are multiple nuclei. Cytoplasm is abundant when one compares it with immature nucleus chromatin pattern. / Velká a lze říci až obrovská nádorová buňka v kostní dření u ALCL ALK negativního T-buněčného lymfomu. Ledvinovitý tvar jádra je spíše typický podobně jako mnohočetná jadérka. Cytoplazma je bohatá při porovnání s nezralým vzhledem jaderného chromatinu. Description: The large cell or one can say almost giant one tumour cell in blood marrow in ALCL and ALK negative T- cell lymphoma. Kidney shape of nucleus is rather typical as also they are multiple nuclei. Cytoplasm is abundant when one compares it with immature nucleus chromatin pattern.
Type: bone marrow
Klíčová slova*: lymphocyte atypical
Anaplastic large cell lymphoma - ALK negative
Another example of anaplastic large lymphoma cell. Here is ex-centric, horse-shoe-shaped nucleus. Eosinophilic area of cytoplasm is at 3 o'clock. Nuclear chromatin is fine, nucleus contains several prominent nucleoli. There is bi-lobar late normoblast with internuclear bride at 3 o'clock. / Jiný příklad buňky anaplastického velkobuněčného lymfomu. Zde je jádro excentrické, podkovovitého tvaru. Eozinofilní oblast cytoplazmy je na 3 hodině. Jaderný chromatin je jemný, jádro obsahuje několik prominujících jadérek. Na 3. hodině je pozdní normoblast, bilobární s jaderným můstkem. Description: Another example of anaplastic large lymphoma cell. Here is ex-centric, horse-shoe-shaped nucleus. Eosinophilic area of cytoplasm is at 3 o'clock. Nuclear chromatin is fine, nucleus contains several prominent nucleoli. There is bi-lobar late normoblast with internuclear bride at 3 o'clock.
Type: bone marrow
Klíčová slova*: lymphocyte atypical
Anaplastic large cell lymphoma - ALK negative
Two lymphoma cells are in the picture. One of them is large with almost round nucleus with fine chromatin and prominent nucleoli, abundant basophilic cytoplasm converts to more basophilic at the right perinuclear area. Another lymphoma cell is smaller (at 8 o'clock) with ex-centric nucleus. It contains fine chromatin and 2 to 3 small nucleoli. Other cells of the picture belong to neutrophil lineage including one mitosis (at the right from the centre). There is one monocyte above at large lymphoma cell. / Na obrázku jsou dvě lymfomové buňky. Jedna z nich je velká s téměř kulatým jádrem, s jemným jaderným chromatinem a prominujícími jadérky, bohatá bazofilní cytoplazma se mění ve více eozinofilní v pravé perinukleární oblasti. Jiná lymfomová buňka (na 8. hodině) je menší s excentrickým jádrem. Obsahuje jemný jaderný chromatin a 2-3 malá jadérka. Další buňky na obrázku patří neutrofilní řadě včetně jedné mitózy (napravo od středu). Jeden monocyt je nad velkou lymfomovou buňkou. Description: Two lymphoma cells are in the picture. One of them is large with almost round nucleus with fine chromatin and prominent nucleoli, abundant basophilic cytoplasm converts to more basophilic at the right perinuclear area. Another lymphoma cell is smaller (at 8 o'clock) with ex-centric nucleus. It contains fine chromatin and 2 to 3 small nucleoli. Other cells of the picture belong to neutrophil lineage including one mitosis (at the right from the centre). There is one monocyte above at large lymphoma cell.
Type: bone marrow
Klíčová slova*: monocyte, lymphocyte atypical
Splenic marginal zone B- cell lymphoma
Four lymphoma cells. The cell at upper left has cleft nucleus or one can say that it is binuclear; chromatin of this cell shows more coarse clumping (more immature) and there is prominent nucleolus at the upper part of the nucleus. / Čtyři lymfomové buňky. Buňka nahoře vlevo má štěpené jádro nebo lze říci, že je binukleární; chromatin této buňky ukazuje více chomáčkovité shlukování (více nezralý) a v horní části jádra je prominující jadérko. Description: Four lymphoma cells. The cell at upper left has cleft nucleus or one can say that it is binuclear; chromatin of this cell shows more coarse clumping (more immature) and there is prominent nucleolus at the upper part of the nucleus.
Type: bone marrow
Klíčová slova*: lymphocyte atypical
Splenic marginal zone B- cell lymphoma
Three lymphoma cells with round or oval nuclei and small nucleoli. Cytoplasmic blebs are evident especially in the cell at the upper right. / Tři lymfomové buňky s kulatými nebo oválnými jádry a malými jadérky. Cytoplazmatické "blebs" jsou zřejmé zejména v buňce nahoře vpravo. Description: Three lymphoma cells with round or oval nuclei and small nucleoli. Cytoplasmic blebs are evident especially in the cell at the upper right.
Type: bone marrow
Klíčová slova*: lymphocyte, lymphocyte atypical
Splenic marginal zone B- cell lymphoma
In bone marrow smears usually villous lymphocytes are less evident than in peripheral blood smears especially when they are made from fresh blood. The picture shows a group of medium size lymphocytes with blue cytoplasm, but cytoplasmic projections are less pronounced. / V kostní dřeni jsou obvykle vilózní lymfocyty méně zřetelné než v nátěrech periferní krve, zvláště jsou-li zhotoveny z čerstvé krve. Obrázek ukazuje skupinu středně velkých lymfocytů s modrou cytoplazmou, ale cytoplazmatické výběžky jsou méně zjevné. Description: In bone marrow smears usually villous lymphocytes are less evident than in peripheral blood smears especially when they are made from fresh blood. The picture shows a group of medium size lymphocytes with blue cytoplasm, but cytoplasmic projections are less pronounced.
Type: bone marrow
Klíčová slova*: lymphocyte atypical
Splenic marginal zone B-cell lymphoma
The most of lymphocytes in the picture belong to lymphoma cells. But that one at 5 o'clock is large granular lymphocyte (LGL) - it means NK cell or mature T lymphocyte. Also lymphocyte at 4 o'clock does not seems to be lymphoma cell. It can be reactive lymphocyte according to pale cytoplasm with more basophilic rim. / Většina lymfocytů na obrázku patří k lymfomovým buňkám. Ale ta na 5. hodině je velký granulovaný lymfocyt (LGL) - to znamená NK buňka či zralý T lymfocyt. Také lymfocyt na 4. hodině se nezdá být buňkou lymfomovou. Může jít o reaktivní lymfocyt podle světlé cytoplazmy s více bazofilním okrajem. Description: The most of lymphocytes in the picture belong to lymphoma cells. But that one at 5 o'clock is large granular lymphocyte (LGL) - it means NK cell or mature T lymphocyte. Also lymphocyte at 4 o'clock does not seems to be lymphoma cell. It can be reactive lymphocyte according to pale cytoplasm with more basophilic rim.
Type: bone marrow
Klíčová slova*: lymphocyte reactive, large granular lymphocyte, lymphocyte atypical
Splenic marginal zone B- cell lymphoma
Massive bone marrow infiltration by lymphoma cells. Their nuclei have condensed chromatin, about half of the cells express small nucleolus. Only very few of the cells are more immature ones (cell in the middle at 3 o'clock) with more immature chromatin and high N/C ratio. Cytoplasm of the most tumour cells have some projections. / Masivní infiltrace kostní dřeně lymfomovými buňkami. Jejich jádra mají kondenzovaný chromatin, asi polovina buněk exprimuje malé jadérko. Jen velmi málo buněk je méně zralých (buňka uprostěd na 3. hodině) s více nezralým chromatinem a vysokým N/C poměrem. Cytoplazma většiny nádorových buněk má nějaké výběžky. Description: Massive bone marrow infiltration by lymphoma cells. Their nuclei have condensed chromatin, about half of the cells express small nucleolus. Only very few of the cells are more immature ones (cell in the middle at 3 o'clock) with more immature chromatin and high N/C ratio. Cytoplasm of the most tumour cells have some projections.
Type: bone marrow
Klíčová slova*: lymphocyte atypical
Splenic marginal zone B- cell lymphoma
Numerous villous lymphocytes in bone marrow smear. Some show nucleoli in their nuclei. There is segmented neutrophil underneath the centre of the picture and one large granular lymphocyte at 5 o' clock. / Početné vilózní lymfocyty v nátěru kostní dřeně. Některé mají jadérka v jádrech. Jeden neutrofilní segment je pod středem obrázku a velký granulovaný lymfocyt na 5. hodině. Description: Numerous villous lymphocytes in bone marrow smear. Some show nucleoli in their nuclei. There is segmented neutrophil underneath the centre of the picture and one large granular lymphocyte at 5 o' clock.
Type: bone marrow
Klíčová slova*: large granular lymphocyte, lymphocyte atypical
Splenic marginal zone B- cell lymphoma
Middle size cells with rather abundant cytoplasm with villi. When one compare there cells with tumour cells of hairy cell leukaemia, they have more basophilic cytoplasm and cytoplasmic projections are more thick and less numerous. / Středně velké buňky se spíše bohatou cytoplazmou s vlásky. Při srovnání těchto buněk s nádorovými buňkami vlasatobuněčné leukémie mají tyto více bazofilní cytoplazmu a cytoplazmatické projekce jsou silnější a méně početné. Description: Middle size cells with rather abundant cytoplasm with villi. When one compare there cells with tumour cells of hairy cell leukaemia, they have more basophilic cytoplasm and cytoplasmic projections are more thick and less numerous.
Type: peripheral blood
Klíčová slova*: lymphocyte atypical
Splenic marginal zone B- cell lymphoma
"Villous lymphocyte" in detail. Rather abundant basophilic cytoplasm with few projections at two opposite sides of the cell. One poorly seen nucleolus is at the lower part of nucleus. Cytoplasm contains some vacuoles and one cytoplasmic inclusion. / "Vilózní lymfocyt" v detailu. Spíše bohatší bazofilní cytoplazma s několika projekcemi na dvou protilehlých stranách buňky. Jedno špatně viditelné jadérko v dolní části jádra. Cytoplazma obsahuje několik vakuol a jednu cytoplazmatickou inkluzi. Description: "Villous lymphocyte" in detail. Rather abundant basophilic cytoplasm with few projections at two opposite sides of the cell. One poorly seen nucleolus is at the lower part of nucleus. Cytoplasm contains some vacuoles and one cytoplasmic inclusion.
Type: peripheral blood
Klíčová slova*: lymphocyte atypical
Splenic marginal zone B-cell lymphoma
In this thinner part of the smear lymphoma cells are better seen in details. Cells have moderately condensed chromatin, nucleoli are rather inconspicuous in this case. Cytoplasm is basophilic with clear projections (two cells at the lower left). / V této tenčí části nátěru jsou lymfomové buňky lépe viditelné v detailech. Buňky mají středně kondenzovaný chromatin, jadérka jsou v tomto případě spíše nezřetelná. Cytoplazma je bazofilní s jasnými projekcemi (dvě buňky vlevo dole). Description: In this thinner part of the smear lymphoma cells are better seen in details. Cells have moderately condensed chromatin, nucleoli are rather inconspicuous in this case. Cytoplasm is basophilic with clear projections (two cells at the lower left).
Type: peripheral blood
Klíčová slova*: lymphocyte atypical
Splenic marginal zone B- cell lymphoma
Another example of "villous lymphocytes" in peripheral blood smear (without EDTA). Cytoplasm of the lymphoma cells is quite basophilic. One segmented eosinophil at the middle left. / Jiný příklad "vilózních lymfocytů" v nátěru periferní krve (bez EDTA). Cytoplazma lymfomových buněk je docela bazofilní. Jeden segmentovaný eozinofil vlevo uprostřed. Description: Another example of "villous lymphocytes" in peripheral blood smear (without EDTA). Cytoplasm of the lymphoma cells is quite basophilic. One segmented eosinophil at the middle left.
Type: peripheral blood
Klíčová slova*: lymphocyte atypical
Splenic marginal zone B- cell lymphoma
Lymphoma cell with short polar villi in peripheral blood smear (fresh capillary blood smear). Cells are larger than normal small lymphocyte with round to oval nucleus. There are not seen clear nucleoli here. / Lymfomové buňky s krátkými polárními výběžky v nátěru periferní krve (nátěr čerstvé kapilární krve). Buňky jsou větší než normální malý lymfocyt, s kulatým až oválným jádrem. Jasná jadérka zde nejsou patrná. Description: Lymphoma cell with short polar villi in peripheral blood smear (fresh capillary blood smear). Cells are larger than normal small lymphocyte with round to oval nucleus. There are not seen clear nucleoli here.
Type: peripheral blood
Klíčová slova*: lymphocyte atypical
Hairy cell leukaemia- BM
Chromatin of nucleus is less condensed to compare normal lymphocyte in hairy cell. Abundant cytoplasm contains few discrete vacuoles and protrudes circumferential in "hairy" projections / Jaderný chromatin "vlasatých" buněk je méně kondenzován ve srování s normálním lymfocytem. Objemná cytoplazma obsahuje několik drobných vakuol a vybíhá do "vlasatých" výběžků po obvodu buňky. Description: Chromatin of nucleus is less condensed to compare normal lymphocyte in hairy cell. Abundant cytoplasm contains few discrete vacuoles and protrudes circumferential in "hairy" projections
Type: bone marrow
Klíčová slova*: hairy cell, lymphocyte atypical, vacuolisation